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fluorescent microscopy nikon eclipse te2000-u  (Nikon)

 
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    Nikon fluorescent microscopy nikon eclipse te2000-u
    Fluorescent Microscopy Nikon Eclipse Te2000 U, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent microscopy nikon eclipse te2000-u/product/Nikon
    Average 90 stars, based on 1 article reviews
    fluorescent microscopy nikon eclipse te2000-u - by Bioz Stars, 2026-02
    90/100 stars

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    Calcium influx and NO production during UV/cold shock-mediated nuclear bubbling. A , B WWOXf HCT116 cells were exposed to UV and then cold shock at 4 °C for 10 min. Increased NO production was shown (stained with green fluorescent DAF). Red arrows indicate the time of initiation of nuclear bubble formation. C Under similar conditions, WWOXd 4T1 cells had little or no increased production of NO. D Calcium influx was observed in UV-treated HCT116 cells (stained with green fluorescent Fluo-8). Calcium chelator EGTA retarded the bubble formation. E HCT116 cells were added 1 μL of DAF for probing Ca 2+ ion (green) and DAPI (blue) and PI (red) for staining nuclei, followed by exposure to UV (960 mJ/cm 2 ) and cold shock at 4 °C for 10 min, and then time-lapse microscopy at 22 °C

    Journal: Cell Communication and Signaling : CCS

    Article Title: Dissociation of the nuclear WWOX/TRAF2 switch renders UV/cold shock-mediated nuclear bubbling cell death at low temperatures

    doi: 10.1186/s12964-024-01866-6

    Figure Lengend Snippet: Calcium influx and NO production during UV/cold shock-mediated nuclear bubbling. A , B WWOXf HCT116 cells were exposed to UV and then cold shock at 4 °C for 10 min. Increased NO production was shown (stained with green fluorescent DAF). Red arrows indicate the time of initiation of nuclear bubble formation. C Under similar conditions, WWOXd 4T1 cells had little or no increased production of NO. D Calcium influx was observed in UV-treated HCT116 cells (stained with green fluorescent Fluo-8). Calcium chelator EGTA retarded the bubble formation. E HCT116 cells were added 1 μL of DAF for probing Ca 2+ ion (green) and DAPI (blue) and PI (red) for staining nuclei, followed by exposure to UV (960 mJ/cm 2 ) and cold shock at 4 °C for 10 min, and then time-lapse microscopy at 22 °C

    Article Snippet: Also, time-lapse bright field and fluorescent microscopy (Nikon Eclipse TE2000-U, Tokyo, Japan) were carried out to image BCD or POD [ , ].

    Techniques: Staining, Time-lapse Microscopy